Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Calregulin expression in non-transfected 293T: (A), mouse Calregulin transfected 293T: (B) and MCF7 (C) whole cell lysates.Application
Western blot analysis of Calregulin expression in HeLa (A) and SK-MEL-28 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | Calregulin Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen (1-3). Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum (4). Calregulin has both low- and high-affinity calcium-binding sites (5). Neither Calnexin nor calregulin contains the calcium-binding “E-F hand” motif found in calmodulins (2). Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Calregulin of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 248-417 mapping at the C-terminus of Calregulin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 248-417 mapping at the C-terminus of Calregulin of human origin |
| Entrez Gene | 811 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Calregulin expression in non-transfected 293T: (A), mouse Calregulin transfected 293T: (B) and MCF7 (C) whole cell lysates.
Application
Western blot analysis of Calregulin expression in HeLa (A) and SK-MEL-28 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Calregulin of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
