Application
Western blot analysis of RABAPTIN-5 expression in non-transfected 293T: (A), human RABAPTIN-5 transfected 293T: (B) and Raji (C) whole cell lysates.Application
Western blot analysis of RABAPTIN-5 expression in HeLa (A) and A-431 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.| Product Name | RABAPTIN-5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 511-810 mapping near the C-terminus of RABAPTIN-5 of human origin |
| Modification Notes | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibits 30-60% homology with Ras p21 (1,2). Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport (1-3). The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function (4-8). Rab proteins are also an integral part of endocytic pathways (5,6). For example, Rab 5 is a potent regulator of endocytic transport that is localized to the plasma membrane, clatherin coated pits and early endosomes (9,10). A possible downstream effector of Rab 5, designated RABAPTIN-5, is a coiled-coil protein that physically associates with Rab 5 (9). Immunodepletion of RABAPTIN-5 strongly inhibits Rab5-dependent early endosome fusion and may be required for membrane docking and fusion (9,10). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RABAPTIN-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 511-810 mapping near the C-terminus of RABAPTIN-5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 511-810 mapping near the C-terminus of RABAPTIN-5 of human origin |
| Entrez Gene | 9135 |
|---|
Application
Western blot analysis of RABAPTIN-5 expression in non-transfected 293T: (A), human RABAPTIN-5 transfected 293T: (B) and Raji (C) whole cell lysates.
Application
Western blot analysis of RABAPTIN-5 expression in HeLa (A) and A-431 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.| Application Notes | recommended for detection of RABAPTIN-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
