Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glioma tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PLC β1 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing membrane and cytoplasmic staining.| Product Name | PLC β1 Antibody (G-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PLC β1 of bovine origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. There are many mammalian PLC isozymes, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC ©1, PLC ©2, PLC ∂1 and PLC ∂2 and PLCe. PLC ∫1, one of the PLC ∫ isozymes, exists as two immunologically distinguishable proteins (PLC-∫1a) and (PLC ∫1b). The two isoforms encode in two distinct transcripts and are generated by alternative splicing of a single gene. PLC ∫1a is preferentially expressed in the cytosol, whereas PLC ∫1b is predominantly localized in the nuclei. PLC ∫1 is a G protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5-triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface. The C-terminal region of PLC ∫1 has Gq GAP activity and has ability to interact with Gq and other PLC-∫1 molecules. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PLC β1 of mouse, rat, human and bovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PLC &beta 1 of bovine origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PLC β1 of bovine origin |
| Entrez Gene | 23236 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glioma tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PLC β1 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing membrane and cytoplasmic staining.| Application Notes | recommended for detection of PLC β1 of mouse, rat, human and bovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
