Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane and cytoplasmic staining.Application
Western blot analysis of PLC γ1 expression in CHO-K1 (A), A-431 (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.| Product Name | PLC γ1 Antibody (530) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 530-850 of PLC γ1 of rat origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC ©1, PLC ©2, PLC ∂1, PLC ∂2 and PLC é. PLC ©1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actin-regulatory protein Villin is tyrosine phosphorylated and associates with PLC ©1 in the brush border of intestinal epithelial cells. Villin regulates PLC ©1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC ©1 binds Integrin å1/∫1 and modulates Integrin å1/∫-specific adhesion. PLC ©1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells. PLC ©1 is rapidly activated in response to growth factor stimulation and plays an important role in regulating cell proliferation and differentiation. It may also have a protective function during cellular response to oxidative stress. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PLC γ1 p145 of mouse, rat, human and hamster origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 530-850 of PLC &gamma 1 of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 530-850 of PLC γ1 of rat origin |
| Entrez Gene | 5335 |
|---|
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane and cytoplasmic staining.
Application
Western blot analysis of PLC γ1 expression in CHO-K1 (A), A-431 (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.| Application Notes | recommended for detection of PLC γ1 p145 of mouse, rat, human and hamster origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
