Application
Western blot analysis of CPI-17 expression in non-transfected: (A) and human CPI-17 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CPI-17 expression in non-transfected: (A) and mouse CPI-17 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing nuclear and cytoplasmic staining of glial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant CPI-17 fusion protein.| Product Name | CPI-17 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 88-147 mapping at the C-terminus of CPI-17 of human origin |
| Modification Notes | CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CPI-17 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 88-147 mapping at the C-terminus of CPI-17 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 88-147 mapping at the C-terminus of CPI-17 of human origin |
| Entrez Gene | 94274 |
|---|
Application
Western blot analysis of CPI-17 expression in non-transfected: (A) and human CPI-17 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CPI-17 expression in non-transfected: (A) and mouse CPI-17 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing nuclear and cytoplasmic staining of glial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant CPI-17 fusion protein.| Application Notes | recommended for detection of CPI-17 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
