Application
Western blot analysis of FTα expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of FTα expression in non-transfected 293T: (A), mouse FTα transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.Application
Western blot analysis of FTα expression in NIH/3T3 (A) and 3611-RF (B) whole cell lysates.| Product Name | FTα Antibody (Y-53) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of FTα of rat origin |
| Modification Notes | Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical a and b subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an ab heterodimer. The b subunit binds the peptide substrate while the a subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate. The a subunit is shared with a second prenyltransferase, geranylgeranyl transferase, that attaches 20 carbon geranylgeranyl to Ras-related proteins that terminate in a Cys-A-A-Xaa recognition site in which Xaa is leucine. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FTα of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of FT&alpha of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of FTα of rat origin |
| Entrez Gene | 2339 |
|---|
Application
Western blot analysis of FTα expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of FTα expression in non-transfected 293T: (A), mouse FTα transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.
Application
Western blot analysis of FTα expression in NIH/3T3 (A) and 3611-RF (B) whole cell lysates.| Application Notes | recommended for detection of FTα of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
