Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear membrane staining of cells in glomeruli and tubules, and cytoplasmic staining of cells in tubules.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing nuclear membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of FRP-3 expression in non-transfected 293T: (A), human FRP-3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of FRP-3 expression in mouse brain tissue extract.| Product Name | FRP-3 Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 156-325 of frizzled-related-protein-3 (FRP-3) of human origin |
| Modification Notes | The frizzled gene, originally identified in Drosophila melanogaster, was shown to be involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins, FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy-terminal Ser/Thr-xxx-Val motif, and they function as receptors for Wnt. Frizzled-1 is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary and in fetal lung and kidney. Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney. The frizzled related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins that contain regions of homology to the cysteine-rich ligand-binding domain of frizzled and a conserved hydrophilic carboxy-terminal. SARP3 is expressed in retinal pigment epithelium (RPE) and pancreas, while expression of FRP-1, 2 and 4 is high in developing tissues. The FRPs/SARPs are involved in the Wnt signaling pathway by regulating the intracellular levels of β-catenin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FRP-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 156-325 of frizzled-related-protein-3 (FRP-3) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 156-325 of frizzled-related-protein-3 (FRP-3) of human origin |
| Entrez Gene | 2487 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear membrane staining of cells in glomeruli and tubules, and cytoplasmic staining of cells in tubules.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing nuclear membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of FRP-3 expression in non-transfected 293T: (A), human FRP-3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of FRP-3 expression in mouse brain tissue extract.| Application Notes | recommended for detection of FRP-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
