Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of TRAF3 expression in TRAF3 transfected (A) and control (B) NIH/3T3 cells.Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and TRAF3 transfected (solid orange histogram) IB4 cells. Dotted pink histogram represents the isotype control, normal goat IgG: .Application
Antibodies tested include TRAF3 (M-20): (A), TRAF3 (C-20): (B) and TRAF3 (M-51): (C).| Product Name | TRAF3 Antibody (M-20) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of TRAF3 of mouse origin |
| Modification Notes | Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily which includes the FAS antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a “TRAF-C” domain that is necessary and sufficient for CD40 binding and homodimerization. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of TRAF3 of mouse and rat origin by WB, IP, IF, IHC(P), FCM and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of TRAF3 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of TRAF3 of mouse origin |
| Entrez Gene | 22031 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of TRAF3 expression in TRAF3 transfected (A) and control (B) NIH/3T3 cells.
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and TRAF3 transfected (solid orange histogram) IB4 cells. Dotted pink histogram represents the isotype control, normal goat IgG: .
Application
Antibodies tested include TRAF3 (M-20): (A), TRAF3 (C-20): (B) and TRAF3 (M-51): (C).| Application Notes | recommended for detection of TRAF3 of mouse and rat origin by WB, IP, IF, IHC(P), FCM and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
