Application
Antibodies tested include p-Shc (73.Tyr 349/350): (A,B) and Shc (H-108): (C,D).Application
Antibodies tested include p-Shc (Tyr 239/240)-R: (A,B) and Shc (H-108): (C,D).Application
Antibodies tested include p-Shc (Tyr 239/240)-R: (A,B,C) and Shc (H-108): (D,E,F).Application
Antibodies tested include p-Shc (Tyr 239): (A,B,C) and Shc (H-108): (D,E,F).Application
Antibodies tested include p-Shc (15E11): (A,B,C) and Shc (H-108): (D,E,F).Application
Antibodies tested include p-Shc (Tyr 317)-R: (A,B,C) and Shc (H-108): (D,E,F).Application
Western blot analysis of Shc expression in non-transfected: (A) and mouse Shc transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Shc expression in SK-BR-3 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed SK-BR-3 cells showing cytoplasmic staining.| Product Name | Shc Antibody (H-108) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 366-473 of the SH2 domain of Shc of human origin |
| Modification Notes | Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus activating mitogenic, as well as other, responses. Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity (1-3). These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate (4-7). Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Shc p66, p52 and p46 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 366-473 of the SH2 domain of Shc of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 366-473 of the SH2 domain of Shc of human origin |
| Entrez Gene | 6464 |
|---|
Application
Antibodies tested include p-Shc (73.Tyr 349/350): (A,B) and Shc (H-108): (C,D).
Application
Antibodies tested include p-Shc (Tyr 239/240)-R: (A,B) and Shc (H-108): (C,D).
Application
Antibodies tested include p-Shc (Tyr 239/240)-R: (A,B,C) and Shc (H-108): (D,E,F).
Application
Antibodies tested include p-Shc (Tyr 239): (A,B,C) and Shc (H-108): (D,E,F).
Application
Antibodies tested include p-Shc (15E11): (A,B,C) and Shc (H-108): (D,E,F).
Application
Antibodies tested include p-Shc (Tyr 317)-R: (A,B,C) and Shc (H-108): (D,E,F).
Application
Western blot analysis of Shc expression in non-transfected: (A) and mouse Shc transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Shc expression in SK-BR-3 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed SK-BR-3 cells showing cytoplasmic staining.| Application Notes | recommended for detection of Shc p66, p52 and p46 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
