Application
Western blot analysis of RGS1 expression in rat intestine extract.| Product Name | RGS1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-70 mapping at the N-terminus of RGS1 of human origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Several Gå GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS 2, RGS4, RGS 7, RGS 9, RGS10 and GAIP (Gå-interacting protein). Each of these proteins has been shown to deactivate specific Gå isoforms by increasing the rate at which they convert GTP to GDP. RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Gå i, Gå o, and Gå t, but not Gå s. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RGS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-70 mapping at the N-terminus of RGS1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-70 mapping at the N-terminus of RGS1 of human origin |
| Entrez Gene | 5996 |
|---|
Application
Western blot analysis of RGS1 expression in rat intestine extract.| Application Notes | recommended for detection of RGS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
