Application
Western blot analysis of VCP expression in NIH/3T3 (A) and 3T3-L1 (B) whole cell lysates.Application
Western blot analysis of VCP expression in non-transfected 293T: (A), human VCP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.Application
Western blot analysis of VCP expression in NIH/3T3 (A), MDA-MB-231 (B), HeLa (C) and MCF7 (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | VCP Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 687-806 mapping at the C-terminus of VCP of human origin |
| Modification Notes | Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated I˚B-å as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of I˚B-å. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of the VCP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 687-806 mapping at the C-terminus of VCP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 687-806 mapping at the C-terminus of VCP of human origin |
| Entrez Gene | 7415 |
|---|
Application
Western blot analysis of VCP expression in NIH/3T3 (A) and 3T3-L1 (B) whole cell lysates.
Application
Western blot analysis of VCP expression in non-transfected 293T: (A), human VCP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.
Application
Western blot analysis of VCP expression in NIH/3T3 (A), MDA-MB-231 (B), HeLa (C) and MCF7 (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of the VCP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
