Application
Antibodies tested include p-GSK-3β (F-2): (A,B) and GSK-3β (H-76): (C,D).Application
Western blot analysis of GSK-3β expression in non-transfected: (A) and mouse GSK-3β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GSK-3β expression in non-transfected: (A) and human GSK-3β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GSK-3β expression in non-transfected 293T: (A), mouse GSK-3β transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of GSK-3β expression in non-transfected 293T: (A), mouse GSK-3β transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of GSK-3β expression in A-431 (A), PDGF-treated A-431 (B), 3611-RF (C) and PDGF-treated 3611-RF (D) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse thymus tissue (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing cytoplasmic localization.Application
Western blot analysis of GSK-3β expression in Jurkat (A) and HeLa (B) whole cell lysates.| Product Name | GSK-3β Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 345-420 mapping at the C-terminus of glycogen synthase kinase-3β of human origin |
| Modification Notes | Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer’s disease. Two forms of GSK-3, designated GSK-3å and GSK-3∫, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer’s disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GSK-3β of mouse, rat, human, rabbit, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 345-420 mapping at the C-terminus of glycogen synthase kinase-3&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 345-420 mapping at the C-terminus of glycogen synthase kinase-3β of human origin |
| Entrez Gene | 2932 |
|---|
Application
Antibodies tested include p-GSK-3β (F-2): (A,B) and GSK-3β (H-76): (C,D).
Application
Western blot analysis of GSK-3β expression in non-transfected: (A) and mouse GSK-3β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GSK-3β expression in non-transfected: (A) and human GSK-3β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GSK-3β expression in non-transfected 293T: (A), mouse GSK-3β transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of GSK-3β expression in non-transfected 293T: (A), mouse GSK-3β transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of GSK-3β expression in A-431 (A), PDGF-treated A-431 (B), 3611-RF (C) and PDGF-treated 3611-RF (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse thymus tissue (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing cytoplasmic localization.
Application
Western blot analysis of GSK-3β expression in Jurkat (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of GSK-3β of mouse, rat, human, rabbit, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
