Application
Western blot analysis of rhophilin expression in non-transfected: (A) and human rhophilin transfected: (B) 293T whole cell lysates.| Product Name | rhophilin Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 119-194 mapping near the N-terminus of rhophilin isoform 3 of human origin |
| Modification Notes | Rho, the Ras-related small GTPase, is responsible for the regulation of Actin-based cytoskeletal structures, including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins act as molecular switches which are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, several proteins have been implicated as Rho effectors. Protein kinase N (PKN) is a fatty acid-activated serine/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino-terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament proteins. A second protein, rhophilin, exhibits 40% sequence identity with the amino-terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron interacts specifically with active Rho and Rac 1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all rhophilin isoforms of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 119-194 mapping near the N-terminus of rhophilin isoform 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 119-194 mapping near the N-terminus of rhophilin isoform 3 of human origin |
| Entrez Gene | 114822 |
|---|
Application
Western blot analysis of rhophilin expression in non-transfected: (A) and human rhophilin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of all rhophilin isoforms of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
