Application
Western blot analysis of Raf-1 expression in non-transfected: (A) and human Raf-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Raf-1 expression in non-transfected: (A) and mouse Raf-1 transfected: (B) 293T whole cell lysates.| Product Name | Raf-1 Antibody (H-71) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 180-250 mapping within an internal region of Raf-1 of human origin |
| Modification Notes | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Raf-1 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 180-250 mapping within an internal region of Raf-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 180-250 mapping within an internal region of Raf-1 of human origin |
| Entrez Gene | 5894 |
|---|
Application
Western blot analysis of Raf-1 expression in non-transfected: (A) and human Raf-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Raf-1 expression in non-transfected: (A) and mouse Raf-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Raf-1 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
