Application
Western blot analysis of Tak1 expression in non-transfected: (A) and mouse Tak1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Tak1 expression in non-transfected 293: (A), human Tak1 transfected 293: (B) and 3611-RF (C) whole cell lysates.Application
Antibodies tested include Tak1 (M-17): (A-C) and Tak1 (M-579): (D-F).| Product Name | Tak1 Antibody (M-579) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-579 representing full length Tak1 (TGFβ-activated kinase) of mouse origin |
| Modification Notes | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a protein with intrinsic kinase activity towards serine/ threonine residues and that is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase sup-pressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGF∫-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGF∫ stimulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Tak1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-579 representing full length Tak1 (TGF&beta -activated kinase) of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-579 representing full length Tak1 (TGFβ-activated kinase) of mouse origin |
| Entrez Gene | 6885 |
|---|
Application
Western blot analysis of Tak1 expression in non-transfected: (A) and mouse Tak1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Tak1 expression in non-transfected 293: (A), human Tak1 transfected 293: (B) and 3611-RF (C) whole cell lysates.
Application
Antibodies tested include Tak1 (M-17): (A-C) and Tak1 (M-579): (D-F).| Application Notes | recommended for detection of Tak1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
