Application
Western blot analysis of PPARα expression in Hep G2 (A) and COLO 320DM (B) nuclear extracts.Application
Antibodies tested include PPARα (H-98): (A) and PPARα (N-19): (B).| Product Name | PPARα Antibody (H-98) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-98 mapping at the N-terminus of PPARα of human origin |
| Modification Notes | Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARå, PPAR∫ (also designated PPAR∂) and PPAR©, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARå is abundant in primary hepatocytes where it regulates the expression of proteins involved in fatty acid metabolism. PPAR∫ is the most widely distributed subtype and is often expressed at high levels. PPAR© is predominantly seen in adipose tissue where it plays a critical role in regulating adipocyte differentiation. Interestingly, both the orphan nuclear hormone receptor LXRå and thyroid receptor (TR) have been shown to act as antagonists of PPARå/RXRå binding to PPREs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of PPARα of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-98 mapping at the N-terminus of PPAR&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-98 mapping at the N-terminus of PPARα of human origin |
| Entrez Gene | 5465 |
|---|
Application
Western blot analysis of PPARα expression in Hep G2 (A) and COLO 320DM (B) nuclear extracts.
Application
Antibodies tested include PPARα (H-98): (A) and PPARα (N-19): (B).| Application Notes | recommended for detection of PPARα of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
