Application
Western blot analysis of ERK 3 expression in KNRK nuclear extract (A) and SJRH30 whole cell lysate (B).Application
Intracellular FCM analysis of fixed and permeabilized A431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .| Product Name | ERK 3 Antibody (H-157) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 565-721 mapping at the C-terminus of ERK 3 of human origin |
| Modification Notes | Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl-2 and caspase-3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK 3 gene maps to chromosome 15q21.2 and encodes a 721 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of ERK 3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 565-721 mapping at the C-terminus of ERK 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 565-721 mapping at the C-terminus of ERK 3 of human origin |
| Entrez Gene | 5597 |
|---|
Application
Western blot analysis of ERK 3 expression in KNRK nuclear extract (A) and SJRH30 whole cell lysate (B).
Application
Intracellular FCM analysis of fixed and permeabilized A431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .| Application Notes | recommended for detection of ERK 3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
