Application
Western blot analysis of human recombinant Nkx-3.1 fusion protein.| Product Name | Nkx-3.1 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Nkx-3.1 of human origin |
| Modification Notes | The homeobox gene Nkx-3.1 is the human homolog of Drosophila bagpipe, which, in conjunction with tinman, determines cell fate in the dorsal mesoderm. In mammalian species, Nkx-3.1 is predominantly expressed in prostate, and it regulates prostate development in response to sonic hedgehog (Shh) signaling by exerting growth-suppressive and differentiating effects on prostatic epithelium. Nkx-3.1 is also expressed at lower levels in other tissues, including the heart and gut, in a Shh independent manner, where it plays a role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate and minor salivary glands. Nkx-3.1 preferentially binds the TAAGTA sequence, which has not been reported for any other NK class homeoprotein. The human Nkx-3.1 gene is located on chromosome 8q21, which frequently undergoes a loss of heterozygosity, and although Nkx-3.1 is not a tumor suppressor gene, it may be a useful marker for benign and malignant prostate epithelium. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Nkx-3.1 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Nkx-3.1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Nkx-3.1 of human origin |
| Entrez Gene | 4824 |
|---|
Application
Western blot analysis of human recombinant Nkx-3.1 fusion protein.| Application Notes | recommended for detection of Nkx-3.1 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
