Application
Western blot analysis of Oct-2 expression in Ramos (A), Daudi (B), Raji (C) and MM-142 (D) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil showing nuclear localization.Application
Western blot analysis of Oct-2 expression in NIH/3T3 nuclear extract.| Product Name | Oct-2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Oct-2 of human origin |
| Modification Notes | POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. The genes Oct-1 and -2 map to human chromosomes 1q22-q23 and 19, respectively . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Oct-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Oct-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Oct-2 of human origin |
| Entrez Gene | 5452 |
|---|
Application
Western blot analysis of Oct-2 expression in Ramos (A), Daudi (B), Raji (C) and MM-142 (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil showing nuclear localization.
Application
Western blot analysis of Oct-2 expression in NIH/3T3 nuclear extract.| Application Notes | recommended for detection of Oct-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
