Application
Western blot analysis of YY1 expression in non-transfected: (A) and human YY1 transfected: (B) 293T whole cell lysates and Jurkat (C) and BJAB (D) nuclear extracts.Application
Western blot analysis of YY1 expression in non-transfected: (A) and mouse YY1 transfected: (B) 293T whole cell lysates and Jurkat (C) and BJAB (D) nuclear extracts.Application
Western blot analysis of YY1 expression in non-transfected 293T: (A), human YY1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of YY1 expression in non-transfected 293T: (A), mouse YY1 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and YY1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with YY1 (H-414): .Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of YY1 expression in Y79 nuclear extract.| Product Name | YY1 Antibody (H-414) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-414 representing full length YY1 of human origin |
| Modification Notes | The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3’ enhancer and immunoglobulin heavy-chain µE1 site as well as the P5 promoter of the adeno-associated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of YY1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-414 representing full length YY1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-414 representing full length YY1 of human origin |
| Entrez Gene | 7528 |
|---|
Application
Western blot analysis of YY1 expression in non-transfected: (A) and human YY1 transfected: (B) 293T whole cell lysates and Jurkat (C) and BJAB (D) nuclear extracts.
Application
Western blot analysis of YY1 expression in non-transfected: (A) and mouse YY1 transfected: (B) 293T whole cell lysates and Jurkat (C) and BJAB (D) nuclear extracts.
Application
Western blot analysis of YY1 expression in non-transfected 293T: (A), human YY1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of YY1 expression in non-transfected 293T: (A), mouse YY1 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and YY1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with YY1 (H-414): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of YY1 expression in Y79 nuclear extract.| Application Notes | recommended for detection of YY1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
