Application
Western blot analysis of T-bet expression in BJAB nuclear extract.Application
Western blot analysis of T-bet expression in non-transfected: (A) and human T-bet transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | T-bet Antibody (H-210) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 326-535 mapping at the C-terminus of T-bet of human origin |
| Modification Notes | T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-©. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of T-bet of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 326-535 mapping at the C-terminus of T-bet of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 326-535 mapping at the C-terminus of T-bet of human origin |
| Entrez Gene | 30009 |
|---|
Application
Western blot analysis of T-bet expression in BJAB nuclear extract.
Application
Western blot analysis of T-bet expression in non-transfected: (A) and human T-bet transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of T-bet of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
