Application
Western blot analysis of HBO1 expression in non-transfected: (A) and human HBO1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HBO1 expression in non-transfected: (A) and mouse HBO1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HBO1 expression in non-transfected: (A) and human HBO1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HBO1 expression in A-673 (A), HeLa (B) and A-431 (C) nuclear extracts.| Product Name | HBO1 Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-180 of HBO1 of human origin |
| Modification Notes | In the cell, transcription is regulated in part by the histone modification of chromatin. Specifically, histone acetyltransferase proteins and their associated complexes function with co-activators to regulate transcription. One family of histone acetyltransferases is the MYST family of transcriptional silencers, which is linked to ORC (origin recognition complex) function. The ORC is an initiator protein for DNA replication and mediates the acetylation of chromatin to control both DNA replication and gene expression. HBO1 (histone acetyltransferase binding to ORC) is a MYST family protein that interacts with ORC1, the largest subunit of the human ORC complex. HBO1 is a nuclear protein that is highly expressed in human testis. In addition to binding ORC, HBO1 represses AR (androgen receptor)-mediated transcription by binding AR through its N-terminal transcriptional repression domain. HBO1 may play a role in regulating AR-dependent gene transcription in normal and prostate cancer cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HBO1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-180 of HBO1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-180 of HBO1 of human origin |
| Entrez Gene | 11143 |
|---|
Application
Western blot analysis of HBO1 expression in non-transfected: (A) and human HBO1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HBO1 expression in non-transfected: (A) and mouse HBO1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HBO1 expression in non-transfected: (A) and human HBO1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HBO1 expression in A-673 (A), HeLa (B) and A-431 (C) nuclear extracts.| Application Notes | recommended for detection of HBO1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
