Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of Importin-7 expression in NTERA-2 cl.D1 (A), IMR-32 (B) and SK-N-MC (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Importin-7 expression in non-transfected 293T: (A), mouse Importin-7 transfected 293T: (B) and MIA PaCa-2 (C) whole cell lysates and IMR-32 (D) and SK-N-MC (E) nuclear extracts.| Product Name | Importin-7 Antibody (H-78) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 961-1038 mapping at the C-terminus of Importin-7 of human origin |
| Modification Notes | Importin-7 (Ran-binding protein 7, RanBP7) is a 1,038 amino acid protein encoded by the human gene IPO7. Importin-7 belongs to the importin b family and contains one importin N-terminal domain. Importin-7 functions in nuclear protein import, either by acting as an autonomous nuclear transport receptor or as an adapter-like protein in association with the Importin b subunit KPNB1. Acting autonomously, Importin-7 is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to Importin-7, the Importin-7/substrate complex dissociates and Importin-7 is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. Importin-7 is a nuclear protein that is expressed in most tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Importin-7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 961-1038 mapping at the C-terminus of Importin-7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 961-1038 mapping at the C-terminus of Importin-7 of human origin |
| Entrez Gene | 10527 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of Importin-7 expression in NTERA-2 cl.D1 (A), IMR-32 (B) and SK-N-MC (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Importin-7 expression in non-transfected 293T: (A), mouse Importin-7 transfected 293T: (B) and MIA PaCa-2 (C) whole cell lysates and IMR-32 (D) and SK-N-MC (E) nuclear extracts.| Application Notes | recommended for detection of Importin-7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
