Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of urothelial cells.Application
Western blot analysis of USF-1 expression in non-transfected: (A) and mouse USF-1 transfected: (B) 293T whole cell lysates.Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with USF-1 (H-86), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear staining of surface epithelial cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and USF-1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with USF-1 (H-86): .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with USF-1 (H-86), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of USF-1 expression in Jurkat nuclear extract.| Product Name | USF-1 Antibody (H-86) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 75-160 mapping within an internal region of USF-1 of human origin |
| Modification Notes | The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of (USF-1) and (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISAGSChIP |
| recommended for detection of USF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 75-160 mapping within an internal region of USF-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 75-160 mapping within an internal region of USF-1 of human origin |
| Entrez Gene | 7391 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of urothelial cells.
Application
Western blot analysis of USF-1 expression in non-transfected: (A) and mouse USF-1 transfected: (B) 293T whole cell lysates.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with USF-1 (H-86), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear staining of surface epithelial cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and USF-1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with USF-1 (H-86): .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with USF-1 (H-86), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of USF-1 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of USF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
