Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.Application
Western blot analysis of GLI-3 expression in K-562 (A), Jurkat (B) and Y79 (C) whole cell lysates.Application
Antibodies tested include GLI-3 (N-19): (A) and GLI-3 (H-280): (B).Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear or cytoplasmic localization.| Product Name | GLI-3 Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-280 of GLI-3 of human origin |
| Modification Notes | It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repressor opposing the effects of hedgehog. Mutations in the ptc gene, which result in unregulated hedgehog signaling, have been correlated with the most common type of cancer, basal cell carcinoma, which affects 750,000 individuals annually in the United States alone. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of GL1-3 of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-280 of GLI-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-280 of GLI-3 of human origin |
| Entrez Gene | 2737 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.
Application
Western blot analysis of GLI-3 expression in K-562 (A), Jurkat (B) and Y79 (C) whole cell lysates.
Application
Antibodies tested include GLI-3 (N-19): (A) and GLI-3 (H-280): (B).
Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear or cytoplasmic localization.| Application Notes | recommended for detection of GL1-3 of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
