Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nucleolar localization.Application
Western blot analysis of UBF expression in SW480 (A), KNRK (B), PC-3 (C), Hep G2 (D) and TH-1 (E) nuclear extracts.Application
Western blot analysis of UBF expression in A-431 nuclear extract.| Product Name | UBF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-220 representing the N-terminus of UBF of human origin |
| Modification Notes | The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex. Two UBF proteins arise from the same gene as a result of alternative mRNA splicings. UBF activity is regulated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues. The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity. Expression of RNA may also be negatively regulated by the two Ku antigens. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of UBF1 and UBF2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-220 representing the N-terminus of UBF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-220 representing the N-terminus of UBF of human origin |
| Entrez Gene | 7343 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nucleolar localization.
Application
Western blot analysis of UBF expression in SW480 (A), KNRK (B), PC-3 (C), Hep G2 (D) and TH-1 (E) nuclear extracts.
Application
Western blot analysis of UBF expression in A-431 nuclear extract.| Application Notes | recommended for detection of UBF1 and UBF2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
