| Product Name | RFC5 Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 161-340 of RFC5 of human origin |
| Modification Notes | Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism including DNA replication, DNA repair, and telomere metabolism (1,2). RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus, and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction (1,3). All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences (1). Four of the five RFC genes (RFC1, RFC2, RFC3, and RFC4) have consensus ATP-binding motifs (4). The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not (5). The human RFC5 gene maps to chromosome 12q24.2-q24.3 and encodes the RFC5 subunit (6). RFC5 and RAD24 are required for DNA damage checkpoint control in the budding yeast Saccharomyces cerevisiae (7). RFC5 is part of a mechanism transducing the DNA damage signal to the activation of the central transducer Rad53 (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RFC5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 161-340 of RFC5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 161-340 of RFC5 of human origin |
| Entrez Gene | 5985 |
|---|
| Application Notes | recommended for detection of RFC5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
