Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lateral ventricle tissue showing nuclear staining of neuronal and non-neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | CENP-F Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2951-3210 of CENP-F of human origin |
| Modification Notes | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Centromere Protein F, CENP-F (also designated mitosin) is a nuclear matrix kinetochore protein that plays a role in mitotic events. In HeLa cells, CENP-F gradually accumulates in the cell cycle, and like CENP-E is preferentially expressed during mitosis where it mediates the G(2) to M phase checkpoint. Upon completion of mitosis, CENP-F is rapidly degraded. CENP-F consists of two coil domains that flank a central flexible core and contains a P-loop (ADIPTGKT) nucleotide binding site in its globular carboxy terminus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CENP-F of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2951-3210 of CENP-F of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2951-3210 of CENP-F of human origin |
| Entrez Gene | 1063 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lateral ventricle tissue showing nuclear staining of neuronal and non-neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of CENP-F of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
