Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of urothelial cells.Application
Antibodies tested include p-Stat2 (Tyr 690)-R: (A,B) and Stat2 (H-190): (C,D).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Stat2 expression in non-transfected 293T: (A), mouse Stat2 transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of Stat2 expression in ZR-75-1 whole cell lysate.| Product Name | Stat2 Antibody (H-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 662-851 mapping at the C-terminus of Stat2 of human origin |
| Modification Notes | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors (1,2). Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex (3). Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3∫ appears to be activated by both while Stat3å is activated by EGF, but not by IL-6 (4,5). Highest expression of Stat4 is seen in testis and myeloid cells (6). IL-12 has been identified as an activator of Stat4 (2). Stat5 has been shown to be activated by prolactin and by IL-3 (7). Stat6 is involved in IL-4 activated signaling pathways (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Stat2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 662-851 mapping at the C-terminus of Stat2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 662-851 mapping at the C-terminus of Stat2 of human origin |
| Entrez Gene | 6773 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of urothelial cells.
Application
Antibodies tested include p-Stat2 (Tyr 690)-R: (A,B) and Stat2 (H-190): (C,D).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Stat2 expression in non-transfected 293T: (A), mouse Stat2 transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of Stat2 expression in ZR-75-1 whole cell lysate.| Application Notes | recommended for detection of Stat2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
