Application
Western blot analysis of Stat6 expression in RAW 264.7 nuclear extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and nuclear staining of urothelial cells.Application
Western blot analysis of Stat6 expression in non-transfected: (A) and human Stat6 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Stat6 expression in NIH/3T3 (A), KNRK (B), RAW 264.7 (C) and BJAB (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.Application
Western blot analysis of Stat6 expression in NIH/3T3 whole cell lysate.| Product Name | Stat6 Antibody (M-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Stat6 of mouse origin |
| Modification Notes | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of proteins that have been designated Stats (signal transducers and activators of transcription. The first members of this family to be described include Stat1å p91, Stat1∫ p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Stat3, which becomes activated in response to epidermal growth factor (EGF) and interleukin-6 (IL-6), but not interferon-© (IFN-©) or Stat4, is an additional member of this family. It has been suggested that the phosphorylated forms of both Stat3 and Stat4 form homodimers as well as heterodimers with the other members of the Stat family, and that differential activation of different Stat proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Other members of the Stat family include Stat5, which has been shown to be activated by prolactin and by IL-3, and Stat6 (also designated IL-4 Stat), which is involved in IL-4-activated signaling pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Stat6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Stat6 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Stat6 of mouse origin |
| Entrez Gene | 6778 |
|---|
Application
Western blot analysis of Stat6 expression in RAW 264.7 nuclear extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and nuclear staining of urothelial cells.
Application
Western blot analysis of Stat6 expression in non-transfected: (A) and human Stat6 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Stat6 expression in NIH/3T3 (A), KNRK (B), RAW 264.7 (C) and BJAB (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.
Application
Western blot analysis of Stat6 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of Stat6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
