Application
Western blot analysis of OXSR1 expression in non-transfected 293: (A), human OXSR1 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of OXSR1 expression in HeLa nuclear extract (A) and A-431 whole cell lysate (B).Application
Western blot analysis of OXSR1 expression in non-transfected: (A) and human OXSR1 transfected: (B) 293T whole cell lysates.| Product Name | OXSR1 Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 351-435 mapping within an internal region of OXSR1 of human origin |
| Modification Notes | Oxidative stress-responsive 1 protein (OXSR1), a protein of 527 amino acids, belongs to the STE20 subfamily. OXSR1 is one of two human homologs of Fray, a serine/threonine kinase expressed in Drosophila. OXSR1 binds to and phosphorylates p21-activated protein kinase PAK1 and regulates downstream kinases in response to environmental stress. Endogenous OXSR1 is activated only by osmotic stresses, notably sorbitol and to a lesser extent NaCl. OXSR1 may also play a role in regulating the actin cytoskeleton. The chloride channel proteins SLC12A1, SLC12A2, and SLC12A6 isoform 2 interact with OXSR1, but SLC12A4 and SLC12A7 do not. The WNK1 and WNK4 protein kinases activate OXSR1 by phosphorlating its T-loop. The OXSR1 protein is widely expressed in mammalian tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of OXSR1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 351-435 mapping within an internal region of OXSR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 351-435 mapping within an internal region of OXSR1 of human origin |
| Entrez Gene | 9943 |
|---|
Application
Western blot analysis of OXSR1 expression in non-transfected 293: (A), human OXSR1 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of OXSR1 expression in HeLa nuclear extract (A) and A-431 whole cell lysate (B).
Application
Western blot analysis of OXSR1 expression in non-transfected: (A) and human OXSR1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of OXSR1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
