Application
Western blot analysis of GADD 153 expression in non-transfected: (A) and mouse GADD 153 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GADD 153 expression in untreated (A) and LPS/PMA-treated (B) RAW 264.7 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.| Product Name | GADD 153 Antibody (F-168) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-168 representing full length GADD 153 of mouse origin |
| Modification Notes | GADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from “classical” C/EBP binding sites, recognizing instead unique “nonclassical” sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GADD 153 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-168 representing full length GADD 153 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-168 representing full length GADD 153 of mouse origin |
| Entrez Gene | 1649 |
|---|
Application
Western blot analysis of GADD 153 expression in non-transfected: (A) and mouse GADD 153 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GADD 153 expression in untreated (A) and LPS/PMA-treated (B) RAW 264.7 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.| Application Notes | recommended for detection of GADD 153 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
