Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Antibodies used include: p27 (M-197): (A) and p27 (N-20): (B).Application
Western blot analysis of p27 expression in non-transfected: (A) and mouse p27 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of p27 expression in non-transfected: (A) and human p27 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of human recombinant p27 fusion protein.| Product Name | p27 Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of p27 of human origin |
| Modification Notes | Cell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of TGF∫-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p27 of mouse, rat, human and mink origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and feline: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of p27 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of p27 of human origin |
| Entrez Gene | 1027 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Antibodies used include: p27 (M-197): (A) and p27 (N-20): (B).
Application
Western blot analysis of p27 expression in non-transfected: (A) and mouse p27 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of p27 expression in non-transfected: (A) and human p27 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of human recombinant p27 fusion protein.| Application Notes | recommended for detection of p27 of mouse, rat, human and mink origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and feline: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
