Application
Immunoperox-idase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and nuclear staining of glandular cells.Application
Western blot analysis of Monoglyceride Lipase expression in non-transfected: (A) and mouse Monoglyceride Lipase transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).| Product Name | Monoglyceride Lipase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 representing full length Monoglyceride Lipase of human origin |
| Modification Notes | Monoglyceride Lipase (MGL), also known as Lysophospholipase-like or Lysophospholipase homolog, is a ubiquitously expressed protein that functions in the endocannabinoid system. It is required for the the degradation of endocannabinoids and the complete hydrolysis of monoglycerides. In addition, Monoglyceride Lipase functions together with HSL (hormone-sensitive lipase) to hydrolyze intracellular triglyceride to glycerol and fatty acids. Monoglyceride Lipase is a presynaptic, cytosolic enzyme that functions as a serine hydrolase and specifically hydrolyzes 2- and 1(3)-ester bonds of monoglycerides. In particular, Monoglyceride Lipase is responsible for the inactivation and degradation of 2-arachidonoylglycerol (2-AG). 2-AG is a monoglyceride produced by neurons that activates cannabinoid receptors and possibly modulates neurotransmitter release and synaptic plasticity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Monoglyceride Lipase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 representing full length Monoglyceride Lipase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 representing full length Monoglyceride Lipase of human origin |
| Entrez Gene | 11343 |
|---|
Application
Immunoperox-idase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and nuclear staining of glandular cells.
Application
Western blot analysis of Monoglyceride Lipase expression in non-transfected: (A) and mouse Monoglyceride Lipase transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).| Application Notes | recommended for detection of Monoglyceride Lipase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
