Application
Western blot analysis of NKRF expression in non-transfected: (A) and human NKRF transfected: (B) 293T whole cell lysates.| Product Name | NKRF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NKRF of human origin |
| Modification Notes | NKRF (NF˚B repressing factor), also known as transcription factor NRF or ITBA4 protein, is a ubiquitously expressed silencer protein. It localizes to the nucleoli but is also found in the nucleoplasm and contains one of each of the following nucleic acid binding domains: G-patch and R3H. NKRF represses the basal transcription of IFN-∫, IL-8 and NOS2 by directly binding to their promoters. NKRF also binds to negative regulatory elements (NREs) and directly interacts with NF˚B via its N-terminus. NKRF specifically inhibits the transcriptional activity of the NF˚B proteins. In addition, NKRF contains a 5’ untranslated region (UTR) that has internal ribosome entry segment (IRES) activity. The activity of the NKRF IRES module is decreased with the down regulation of the RNA-binding protein, JKTBP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NKRF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NKRF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NKRF of human origin |
| Entrez Gene | 55922 |
|---|
Application
Western blot analysis of NKRF expression in non-transfected: (A) and human NKRF transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of NKRF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
