Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Antibodies tested include p-FADD (Ser 194): (A,B) and FADD (H-181): (C,D).Application
Western blot analysis of FADD expression in non-transfected 293T: (A), mouse FADD transfected 293T: (B) and SW480 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular and lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of FADD expression in non-transfected: (A) and mouse FADD transfected: (B) 293T whole cell lysates.Application
Blots were probed with FADD (H-181): (A) and p-FADD (Ser 194)-R: (B,C). In B and C, the antibody was preincubated with cognate non-phosphorylated or phosphorylated peptide, respectively.Application
Antibodies tested include FADD (H-181): (A,B) and p-FADD (Ser 194): (C,D).Application
Western blot analysis of human recombinant FADD expression in A-431 cells.| Product Name | FADD Antibody (H-181) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 28-209 mapping at the C-terminus of FADD of human origin |
| Modification Notes | In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved "death domain" and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-R1 interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-R1-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-R1 via their DDH regions. Overexpression of TRADD leads to NFkB activation and apoptosis in the absence of TNF. Overexpression of FADD causes apoptosis, which can be blocked by the cow pox protein CrmA, suggesting that FADD lies upstream of ICE and possibly other serine proteases. The receptor interacting protein, RIP, associates with FAS exclusively via its DDH and this association is abrogated in lpr mutants. Unlike TRADD and FADD, RIP contains a putative amino terminal kinase domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FADD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 28-209 mapping at the C-terminus of FADD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 28-209 mapping at the C-terminus of FADD of human origin |
| Entrez Gene | 8772 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Antibodies tested include p-FADD (Ser 194): (A,B) and FADD (H-181): (C,D).
Application
Western blot analysis of FADD expression in non-transfected 293T: (A), mouse FADD transfected 293T: (B) and SW480 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular and lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of FADD expression in non-transfected: (A) and mouse FADD transfected: (B) 293T whole cell lysates.
Application
Blots were probed with FADD (H-181): (A) and p-FADD (Ser 194)-R: (B,C). In B and C, the antibody was preincubated with cognate non-phosphorylated or phosphorylated peptide, respectively.
Application
Antibodies tested include FADD (H-181): (A,B) and p-FADD (Ser 194): (C,D).
Application
Western blot analysis of human recombinant FADD expression in A-431 cells.| Application Notes | recommended for detection of FADD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
