Application
Western blot analysis of DOT1L1 expression in non-transfected: (A) and mouse DOT1L1 transfected: (B) 293T whole cell lysates.| Product Name | DOT1L1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminal of DOT1L1 of human origin |
| Modification Notes | DOT1L1, also known as DOT1L (DOT1-like, histone H3 methyltransferase), DOT1 or KMT4, is a 1739 amino acid homolog of the yeast DOT1 (disruptor of telomeric silencing-1) protein. Localized to the nucleus and highly expressed in testis, lung and kidney, DOT1L1 is a histone methyltransferase that transfers methyl groups from S-adenosyl-L-methionine to lysine residues on various substrates, such as nucleosomes or histones. While most histone methyltransferases contain a SET domain through which they confer their enzymatic activity, DOT1L1 does not contain this characteristic domain and is, therefore, thought to function through a different mechanism. DOT1L1 can bind with several MLL-fusion partners found in acute leukemia and, through this binding, can promote oncogenesis. Two isoforms of DOT1L1 are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DOT1L1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminal of DOT1L1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminal of DOT1L1 of human origin |
| Entrez Gene | 84444 |
|---|
Application
Western blot analysis of DOT1L1 expression in non-transfected: (A) and mouse DOT1L1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of DOT1L1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
