Application
Western blot analysis of HADHSC expression in HEK293 (A), Hep G2 (B), WiDR (C) and MCF7 (D) whole cell lysates and mouse spleen (E) and human kidney (F) tissue extracts.Application
Western blot analysis of HADHSC expression in HEK293 (A) and Hep G2 (B) whole cell lysates.| Product Name | HADHSC Antibody (H-164) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 151-314 mapping at the C-terminus of HADHSC of human origin |
| Modification Notes | HADHSC (hydroxyacyl-Coenzyme A (CoA) dehydrogenase, short chain), also known as HAD, HHF4, HADH1, SCHAD or M/SCHAD (Medium and short chain L-3-hydroxyacyl-CoA dehydrogenase), is a mitochondrial matrix protein expressed in pancreas, liver, heart, kidney and skeletal muscle. HADHSC exists as a homodimer that participates in lipid metabolism and is essential for the ∫-oxidation of medium and short chain fatty acids. More specifically, HADHSC catalyzes the dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs while NAD+ is simultaneously reduced to NADH. Defects in HADHSC can lead to HADH (3-å-hydroxyacyl-CoA dehydrogenase) deficiency and familial hyperinsulinemic hypoglycemia 4 (HHF4). HADH deficiency is characterized as a metabolic disorder with patients exhibiting hepatoencephalopathy, hypoglycemia, myopathy or cardiomyopathy and sometimes experiencing sudden death. HHF4 is a disorder characterized by elevated insulin secretion that, if left untreated, can cause brain damage from recurrent hypoglycemia episodes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HADHSC of mouse, rat, equine and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 151-314 mapping at the C-terminus of HADHSC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 151-314 mapping at the C-terminus of HADHSC of human origin |
| Entrez Gene | 3033 |
|---|
Application
Western blot analysis of HADHSC expression in HEK293 (A), Hep G2 (B), WiDR (C) and MCF7 (D) whole cell lysates and mouse spleen (E) and human kidney (F) tissue extracts.
Application
Western blot analysis of HADHSC expression in HEK293 (A) and Hep G2 (B) whole cell lysates.| Application Notes | recommended for detection of HADHSC of mouse, rat, equine and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
