Application
Western blot analysis of TRH expression in A549 whole cell lysate.| Product Name | TRH Antibody (A-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of TRH of human origin |
| Modification Notes | TRH (thyrotrophin-releasing hormone), also known as TRF, Prothyroliberin or Thyroliberin, is a 242 amino acid secreted protein that belongs to the TRH family and is expressed in the hypothalamus in response to hypothyroidism. TRH is a hypothalamic tripeptide that stimulates the release of thyrotrophin (TSH) and prolactin through its receptor in the anterior pituitary gland. In addition to regulating the biosynthesis of TSH, TRH acts as a neurotransmitter and a neuromodulator in the central and peripheral nervous systems. An integral membrane ectopeptidase protein, PGP-II (pyroglutamyl peptidase II), cleaves and inactivates TRH in neural and hormonal transmissions. TRH may function as a melanoma autocrine growth factor in melanomas and dysplastic nevi. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRH of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of TRH of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of TRH of human origin |
| Entrez Gene | 7200 |
|---|
Application
Western blot analysis of TRH expression in A549 whole cell lysate.| Application Notes | recommended for detection of TRH of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
