Application
Western blot analysis of LDH-D expression in non-transfected: (A) and mouse LDH-D transfected: (B) 293T whole cell lysates and mouse heart (C) and mouse skeletal muscle (D) tissue extracts.Application
Western blot analysis of LDH-D expression in LADMAC whole cell lysate.| Product Name | LDH-D Antibody (H-195) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-195 mapping at the N-terminus of LDH-D of human origin |
| Modification Notes | The lactate dehydrogenase family (LDH) consists of three members, designated LDH-A, LDH-B and LDH-C, all of which work in concert to catalyze the final step of anaerobic glycolysis, namely the conversion of L-lactate and NAD+ to pyruvate and NADH. Each family member displays a specific tissue distribution pattern, with LDH-A present in muscle and LDH-B present in heart, while LDH-C expression is confined to testis and sperm. A fourth possible member, termed LDH-D (lactate dehydrogenase D), probable D-lactate dehydrogenaseor DLD, is a 507 amino acid mitochondrial protein belonging to the D-isomer specific 2-hydroxyacid dehydrogenase family. Existing as two alternatively spliced isoforms, LDH-D is moderately expressed in liver and heart with lower levels found in kidney and skeletal muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LDH-D of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-195 mapping at the N-terminus of LDH-D of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-195 mapping at the N-terminus of LDH-D of human origin |
Application
Western blot analysis of LDH-D expression in non-transfected: (A) and mouse LDH-D transfected: (B) 293T whole cell lysates and mouse heart (C) and mouse skeletal muscle (D) tissue extracts.
Application
Western blot analysis of LDH-D expression in LADMAC whole cell lysate.| Application Notes | recommended for detection of LDH-D of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
