Application
Western blot analysis of D54 expression in non-transfected: (A) and human D54 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of D54 expression in non-transfected 293T: (A), mouse D54 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | D54 Antibody (L-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of D54 of human origin |
| Modification Notes | The tumor protein D52 (TPD52) family consists of three members, TPD52, TPD52L1 (D53), and TPD52L2 (D54). These small coiled-coil motif bearing proteins interact in hetero- and homomeric fashion, and have been implicated in cell proliferation, apoptosis, and vesicle trafficking. The TPD52 gene maps to chromosome 8q12, and due to amplification, shows frequent overexpression in prostate and breast carcinomas. D54 is a 206 amino acid protein that is often co-expressed with TPD52 in the bone marrow of patients with ALL and AML, suggesting that it has the potential to play a role in malignancy. D54 interacts with MAL2, a member of the polarized machinery transport system that is required for transcytosis, a transporter pathway that is used to deliver membrane-bound cargo from perinuclear endosomes to the apical surface in a raft-dependent manner. There are three isoforms of D54 that are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of D54 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of D54 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of D54 of human origin |
| Entrez Gene | 7165 |
|---|
Application
Western blot analysis of D54 expression in non-transfected: (A) and human D54 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of D54 expression in non-transfected 293T: (A), mouse D54 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of D54 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
