Application
Western blot analysis of XRN2 expression in NTERA-2 cl.D1 (A) and DU 145 (B) whole cell lysates.Application
Western blot analysis of XRN2 expression in non-transfected: (A) and human XRN2 transfected: (B) 293T whole cell lysates.| Product Name | XRN2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 651-950 mapping at the C-terminus of XRN2 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Degradation of mRNA is a critical aspect of gene expression that occurs via the exoribonuclease. Exoribonuclease 2 (XRN2) is the human homologue of the Saccharomyces cerevisiae RAT1, which functions as a nuclear 5' to 3' exoribonuclease and is essential for mRNA turnover and cell viability. XRN2 also processes rRNAs and small nucleolar RNAs (snoRNAs) in the nucleus. XRN2 moves along with RNA polymerase II and gains access to the nascent RNA transcript after the endonucleolytic cleavage at the poly(A) site or at a second cotranscriptional cleavage site (CoTC). CoTC is an autocatalytic RNA structure that undergoes rapid self-cleavage and acts as a precursor to termination by presenting a free RNA 5' end to be recognized by XRN2. XRN2 then travels in a 5'-3' direction like a guided torpedo and facilitates the dissociation of the RNA polymerase elongation complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 ELISA:1:1000~1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine, bovine and porcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 651-950 mapping at the C-terminus of XRN2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 651-950 mapping at the C-terminus of XRN2 of human origin |
Application
Western blot analysis of XRN2 expression in NTERA-2 cl.D1 (A) and DU 145 (B) whole cell lysates.
Application
Western blot analysis of XRN2 expression in non-transfected: (A) and human XRN2 transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 ELISA:1:1000~1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
