Application
Western blot analysis of Kell expression in non-transfected: (A) and human Kell transfected: (B) 293T whole cell lysates.| Product Name | Kell Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-380 mapping near the N-terminus of Kell of human origin |
| Modification Notes | The KEL (CD238) gene encodes a type II transmembrane endopeptidase, Kell, that shares a consensus sequence with a large family of zinc-dependent endopeptidases. The Kell blood group protein is expressed primarily in the erythroid tissues and testis and with weaker expression in a large number of other tissues such as brain and lymphoid tissues. Immunohistochemistry reveals human Kell protein is localized to the Sertoli cells of the testis and the follicular dendritic cells of the spleen and tonsil. Kell is one of the major human surface antigens on red blood cells where it is linked by a single disulfide bond to XK. The absence of XK, as occurs in the McLeod phenotype, is associated with a set of clinical symptoms that include nerve and muscle disorders and red cell acanthocytosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Kell (also designated CD238) of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-380 mapping near the N-terminus of Kell of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-380 mapping near the N-terminus of Kell of human origin |
| Entrez Gene | 3792 |
|---|
Application
Western blot analysis of Kell expression in non-transfected: (A) and human Kell transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Kell (also designated CD238) of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
