| Product Name | AFF4 Antibody (H-189) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 65-253 mapping near the N-terminus of AFF4 of human origin |
| Modification Notes | AFF4 (AF4/FMR2 family, member 4), also known as MCEF or AF5Q31, localizes to the nucleus and is a member of the AF4 family of transcription factors. Ubiquitously expressed with highest expression in placenta, heart, pancreas and skeletal muscle, AFF4 is a 1,163 amino acid component of the positive transcription elongation factor b (P-TEFb) complex that contains Cdk9 (cyclin-dependent kinase 9) and cyclin T1. AFF4 is thought to function as a transcription factor that positively regulates transcription during fetal development, as well as in adult tissue. Defects in the gene encoding AFF4 lead to expression of an MLL-AFF4 (myeloid/lymphoid or mixed-lineage leukemia-AFF4) fusion protein that is found in acute lymphoblastic leukemia (ALL), implicating AFF4 in the pathogenesis of ALL. Three isoforms of AFF4 are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AFF4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 65-253 mapping near the N-terminus of AFF4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 65-253 mapping near the N-terminus of AFF4 of human origin |
| Entrez Gene | 27125 |
|---|
| Application Notes | recommended for detection of AFF4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
