Application
Western blot analysis of WRP expression in non-transfected: (A) and human WRP transfected: (B) 293T whole cell lysates.| Product Name | WRP Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 821-970 mapping near the C-terminus of WRP of human origin |
| Modification Notes | WAVE-associated Rac GTPase-activating protein (WRP), also known as SLIT-ROBO Rho GTPase-activating protein 3 (srGAP3) and Mental disorder-activating GAP (MEGAP), is a 1099 amino acid protein containing 1 FCH domain, 1 Rho-GAP domain and 1 SH3 domain. Expressed highly in brain, and in lower levels in kidney, WRP is thought to play a role in cell migration through its interaction with Cdc42 and Rac1. Cdc42 and Rac1 are two intracellular signaling proteins that regulate the multistep cell migration process. WRP downregulates Cdc42 and Rac1 activity, thereby impairing actin and microtubule dynamics, the formation of protrusions, and total cell migration. Defects in the gene encoding WRP have been linked to severe idiopathic mental retardation. Three isoforms of WRP exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of WRP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 821-970 mapping near the C-terminus of WRP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 821-970 mapping near the C-terminus of WRP of human origin |
| Entrez Gene | 9901 |
|---|
Application
Western blot analysis of WRP expression in non-transfected: (A) and human WRP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of WRP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
