Application
Western blot analysis of PSTK expression in non-transfected: (A) and human PSTK transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PSTK expression in non-transfected: (A) and mouse PSTK transfected: (B) 293T whole cell lysates.| Product Name | PSTK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PSTK of human origin |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. PSTK (phosphoseryl-tRNA kinase), also known as L-seryl-tRNA(Sec) kinase, is a 348 amino acid enzyme belonging to the L-seryl-tRNA(Sec) kinase family. An essential RNA-dependent kinase, PSTK plays a role in aminoacyl-tRNA synthesis and the biosynthesis of selenocysteine, the 21st natural amino acid. Utilizing magnesium as a cofactor, PSTK converts seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), the immediate precursor of selenocysteinyl-tRNA(Sec). PSTK exists as two alternatively spliced isoforms and is encoded by a gene mapping to human chromosome 10q26.13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PSTK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PSTK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PSTK of human origin |
| Entrez Gene | 118672 |
|---|
Application
Western blot analysis of PSTK expression in non-transfected: (A) and human PSTK transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PSTK expression in non-transfected: (A) and mouse PSTK transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PSTK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
