Application
Western blot analysis of DDO expression in non-transfected: (A) and mouse DDO transfected: (B) 293T whole cell lysates.| Product Name | DDO Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 151-320 mapping near the C-terminus of DDO of human origin |
| Modification Notes | DDO (D-aspartate oxidase), also known as DASOX, is a 341 amino acid protein that localizes to peroxisomes and exists as two alternatively spliced isoforms, designated DDO-1 and DDO-2. Using FAD or 6-hydroxyflavin adenine dinucleotide as cofactors, DDO functions as a peroxisomal flavoprotein that selectively catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Human DDO shares 86% sequence similarity with its bovine counterpart, suggesting a conserved role between species. The gene encoding DDO maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DDO of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 151-320 mapping near the C-terminus of DDO of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 151-320 mapping near the C-terminus of DDO of human origin |
| Entrez Gene | 8528 |
|---|
Application
Western blot analysis of DDO expression in non-transfected: (A) and mouse DDO transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of DDO of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
