Application
Western blot analysis of HOOK2 expression in non-transfected: (A) and human HOOK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HOOK2 expression in non-transfected: (A) and human HOOK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HOOK2 expression in Hep G2 whole cell lysate.Application
Western blot analysis of HOOK2 expression in non-transfected: (A) and human HOOK2 transfected: (B) 293T whole cell lysates.| Product Name | HOOK2 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 157-236 mapping within an internal region of HOOK2 of human origin |
| Modification Notes | Microtubules mediate the spatial organization of diverse membrane-trafficking systems. The HOOK proteins, HOOK1, HOOK2 and HOOK3, comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules; and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 (also known as HK2) is 719 amino acids in length. It exists as a homodimer, most likely mediated through its central coiled-coil domain. HOOK2 may associate with SURF1 and Zic2, and all three may be potential esophageal cancer tumor antigens. HOOK2 expression is strong in the larynx and the esophagus. Unlike HOOK3, which localizes to the Golgi, HOOK2 localizes to discrete subcellular structures not corresponding to early or late endosomes, mitochondria, Golgi complex, endoplasmic reticulum, lysosomes or multivesicular bodies. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HOOK2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 157-236 mapping within an internal region of HOOK2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 157-236 mapping within an internal region of HOOK2 of human origin |
| Entrez Gene | 29911 |
|---|
Application
Western blot analysis of HOOK2 expression in non-transfected: (A) and human HOOK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HOOK2 expression in non-transfected: (A) and human HOOK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HOOK2 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of HOOK2 expression in non-transfected: (A) and human HOOK2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HOOK2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
