Application
Western blot analysis of PIPOX expression in non-transfected: (A) and mouse PIPOX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PIPOX expression in non-transfected 293T: (A), human PIPOX transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Product Name | PIPOX Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 176-345 mapping near the C-terminus of PIPOX of human origin |
| Modification Notes | PIPOX (pipecolic acid oxidase), also known as LPIPOX or PSO, is a 390 amino acid protein that localizes to the peroxisome and belongs to the MSOX/MTOX family. Existing as a monomer, PIPOX uses FAD as a cofactor to catalyze the metabolism and subsequent degradation of sarcosine, L-pipecolic acid and L-proline. The gene encoding PIPOX maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PIPOX of mouse and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 176-345 mapping near the C-terminus of PIPOX of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 176-345 mapping near the C-terminus of PIPOX of human origin |
| Entrez Gene | 51268 |
|---|
Application
Western blot analysis of PIPOX expression in non-transfected: (A) and mouse PIPOX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PIPOX expression in non-transfected 293T: (A), human PIPOX transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of PIPOX of mouse and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
