Application
Western blot analysis of LRP5 expression in non-transfected: (A) and mouse LRP5 transfected: (B) 293T whole cell lysates.| Product Name | LRP5 Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1371-1475 mapping near the C-terminus of LRP5 of human origin |
| Modification Notes | Members of the LDL receptor gene family, including LDLR (low density lipoprotein receptor), LRPs (low density lipoprotein related proteins), Megalin (also designated GP330), VLDLR (very low density lipoprotein receptor) and ApoER2, are characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain (1,2). Of the known family members, LRP5 is most closely related to LRP1 (3). However, LRP5 has a unique organization of EGF and LDLR repeats compared to other LDLR family members and likely represents a new category in this family (3). LRP is expressed in rat tibia in areas of the bone that are involved in remodeling (4). LRP5 is a Wnt coreceptor that binds to Axin and regulates the canonical Wnt signaling pathway (5). LRP5 affects bone mass accrual during growth and mutations in LRP5 cause the autosomal recessive disorder osteoporosis-pseudoglioma syndrome (OPPG) (6). The gene which encodes LRP5 maps to human chromosome 11q13.4 (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LRP5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1371-1475 mapping near the C-terminus of LRP5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1371-1475 mapping near the C-terminus of LRP5 of human origin |
Application
Western blot analysis of LRP5 expression in non-transfected: (A) and mouse LRP5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of LRP5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
